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SiRNA Validation Data

siRNA ID:

117160

Gene ID:

7378

Transcript(s):

NM_001287426.1 NM_001287428.1 NM_001287429.1 NM_001287430.1 NM_003364.3 NR_109837.1 XM_005249838.4 XM_011515512.2 XM_011515513.2 XM_011515514.2 XM_011515515.2

Validation Results:

13%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00427695_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.