SiRNA Validation Data

siRNA ID:

119195

Gene ID:

Transcript(s):

NM_001303486.1 NM_001303487.1 NM_001324381.1 NM_003631.3 NR_130168.1 NR_136752.1 NR_136754.1 NR_136755.1 NR_136756.1 XM_011540305.1 XM_011540308.2

Validation Results:

6%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00608254_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.