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SiRNA Validation Data

siRNA ID:

119406

Gene ID:

85007

Transcript(s):

NM_001278346.1 NM_153373.3 NR_103508.1 NR_103509.1 NR_103510.1 XM_006714925.2 XM_011534669.2 XM_011534670.2 XM_011534671.2 XM_011534672.2 XM_011534674.2 XM_011534676.2 XM_017009996.1 XM_017009997.1 XM_017009998.1 XM_017009999.1

Validation Results:

16%(+/-8% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00538025_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.