SiRNA Validation Data

siRNA ID:

119781

Gene ID:

Transcript(s):

NM_001112.3 NM_001160230.1 NM_015833.3 NM_015834.3 NR_027673.1 NR_027674.1 NR_073200.1 XM_006723954.2 XM_011529425.2 XM_017028242.1 XM_017028243.1 XM_017028244.1 XM_017028245.1 XM_017028246.1 XM_017028247.1 XM_017028248.1 XM_017028249.1 XM_017028250.1 XM_017028251.1 XM_017028252.1 XM_017028253.1 XM_017028254.1 XM_017028255.1 XM_017028256.1

Validation Results:

8%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00210562_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.