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SiRNA Validation Data

siRNA ID:

s10543

Gene ID:

5298

Transcript(s):

NM_001198773.1 NM_001198774.1 NM_001198775.1 NM_002651.2 XM_005245264.3 XM_011509634.1 XM_011509635.1 XM_011509636.2 XM_011509637.2 XM_011509638.1 XM_017001486.1 XM_017001487.1 XM_017001488.1 XM_017001489.1 XM_017001490.1 XM_017001491.1 XM_017001492.1

Validation Results:

5%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00356327_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer^® Select siRNA or 5 nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.