SiRNA Validation Data

siRNA ID:

s21042

Gene ID:

10713

Transcript(s):

NM_001256725.1 NM_001256726.1 NM_001256727.1 NM_001256728.1 NM_006590.3 NR_046347.1 XM_006711922.1 XM_006711923.2 XM_011532487.1 XM_011532488.1 XM_017003182.1

Validation Results:

14%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00198180_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.