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SiRNA Validation Data

siRNA ID:

s224151

Gene ID:

5031

Transcript(s):

NM_001277204.1 NM_001277205.1 NM_001277206.1 NM_001277207.1 NM_001277208.1 NM_176796.2 NM_176797.2 NM_176798.2 XM_005274022.3 XM_006718571.3 XM_011545076.2 XM_011545077.2 XM_011545079.2

Validation Results:

2%(+/-0% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00602548_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.