Close X

SiRNA Validation Data

siRNA ID:

s45

Gene ID:

4705

Transcript(s):

NM_001322019.1 NM_001322020.1 NM_004544.3 NR_136155.1 NR_136156.1 NR_136157.1 NR_136158.1 XM_011511228.2

Validation Results:

13%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00190004_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.