Close X

SiRNA Validation Data

siRNA ID:

1024

Gene ID:

55351

Transcript(s):

NM_001306082.1 NM_018401.2 XM_005247982.3 XM_005247983.4 XM_011513497.1 XM_011513501.2 XM_011513504.1 XM_017008377.1

Validation Results:

12%(+/-0% mRNA remaining)(HeLa cell line)

Validation Procedure:

The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.