SiRNA Validation Data

siRNA ID:

105162

Gene ID:

Transcript(s):

NM_001283041.1 NM_001283042.1 NM_013396.4 XM_006723992.3 XM_011529529.1 XM_011529531.2 XM_011529533.2 XM_011529535.2 XM_017028321.1 XM_017028322.1 XM_017028323.1 XM_017028324.1 XM_017028325.1 XM_017028327.1 XM_017028328.1 XM_017028329.1

Validation Results:

23%(+/-8% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00203650_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.