SiRNA Validation Data

siRNA ID:

1075

Gene ID:

Transcript(s):

NM_001102406.1 NM_001253884.1 NM_025144.3 XM_005263245.4 XM_005263246.4 XM_006714326.3 XM_017008633.1 XM_017008634.1 XM_017008635.1 XM_017008636.1 XM_017008637.1 XM_017008638.1 XM_017008639.1 XM_017008640.1 XM_017008641.1 XM_017008642.1 XM_017008643.1 XM_017008644.1 XM_017008645.1 XM_017008646.1 XM_017008647.1 XM_017008648.1 XM_017008649.1 XM_017008650.1 XM_017008651.1

Validation Results:

9%(+/-0% mRNA remaining)(HeLa cell line)

Validation Procedure:

The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.