SiRNA Validation Data

siRNA ID:

11207

Gene ID:

Transcript(s):

NM_001159508.1 NM_002225.3 XM_005254350.2 XM_006720492.2 XM_006720495.2 XM_017022149.1 XM_017022150.1 XM_017022151.1 XM_017022152.1 XM_017022153.1 XM_017022154.1 XM_017022155.1 XM_017022156.1 XM_017022157.1 XM_017022158.1

Validation Results:

20%(+/-8% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00158370_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.