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SiRNA Validation Data

siRNA ID:

118630

Gene ID:

896

Transcript(s):

NM_001136017.3 NM_001136125.2 NM_001136126.2 NM_001287427.1 NM_001287434.1 NM_001760.4 XM_011514971.1 XM_011514972.1

Validation Results:

11%(+/-5% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00426901_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.