SiRNA Validation Data

siRNA ID:

118721

Gene ID:

Transcript(s):

NM_001282236.1 NM_004196.4 XM_005268157.2 XM_005268159.2 XM_005268160.4 XM_011537276.1 XM_017021729.1 XM_017021730.1 XM_017021731.1 XM_017021732.1

Validation Results:

13%(+/-5% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00177766_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.