85671456
NM_001135943.1 NM_001135944.1 NM_003682.3 NM_130470.2 NM_130471.2 NM_130472.2 NM_130473.2 NM_130474.2 NM_130475.2 NM_130476.2 XM_005253189.2 XM_005253196.2 XM_005253199.2 XM_005253200.2 XM_005253201.2 XM_005253203.2 XM_005253204.2 XM_005253205.1 XM_017018478.1 XM_017018479.1 XM_017018480.1 XM_017018481.1 XM_017018482.1 XM_017018483.1 XM_017018484.1 XM_017018485.1 XM_017018486.1 XM_017018487.1 XM_017018488.1 XM_017018489.1 XM_017018490.1 XM_017018491.1 XM_017018492.1 XM_017018493.1 XM_017018494.1 XM_017018495.1 XM_017018496.1 XM_017018497.1 XM_017018498.1 XM_017018499.1 XM_017018500.1 XM_017018501.1 XM_017018502.1 XM_017018503.1 XM_017018504.1 XM_017018505.1 XM_017018506.1 XM_017018507.1 XM_017018508.1 XM_017018509.1 XM_017018510.1
30%(+/-0% mRNA remaining)(HeLa cell line)
The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.