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SiRNA Validation Data

siRNA ID:

262

Gene ID:

5163

Transcript(s):

NM_001278549.1 NM_002610.4 NR_103729.1 NR_103731.1 XM_006712594.2 XM_006712595.3 XM_011511343.2 XM_011511344.2 XM_011511345.2 XM_011511347.2

Validation Results:

23%(+/-0% mRNA remaining)(HeLa cell line)

Validation Procedure:

The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.