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SiRNA Validation Data

siRNA ID:

34305

Gene ID:

153090

Transcript(s):

NM_032552.3 NM_138709.2 XM_005251719.4 XM_005251721.1 XM_005251724.4 XM_011518264.2 XM_011518265.2 XM_011518266.2 XM_011518267.2 XM_011518270.2 XM_011518271.2 XM_017014298.1 XM_017014299.1 XM_017014300.1

Validation Results:

9%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00368995_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.