SiRNA Validation Data

siRNA ID:

45

Gene ID:

5213

Transcript(s):

NM_000289.5 NM_001166686.1 NM_001166687.1 NM_001166688.1 XM_005268974.1 XM_005268975.1 XM_005268976.3 XM_005268977.1 XM_005268978.2 XM_005268979.1 XM_011538487.1 XM_011538488.2 XM_017019467.1 XM_017019468.1 XM_017019469.1

Validation Results:

27%(+/-0% mRNA remaining)(HeLa cell line)

Validation Procedure:

The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.