SiRNA Validation Data

siRNA ID:

803

Gene ID:

7444

Transcript(s):

NM_001130480.2 NM_001130481.2 NM_001130482.2 NM_001130483.2 NM_001288836.1 NM_001288837.1 NM_001288838.1 NM_001288839.1 NM_006296.6 XM_005264540.4 XM_006712090.3 XM_006712091.3 XM_006712092.3 XM_011533092.2 XM_017004858.1 XM_017004859.1 XM_017004860.1 XM_017004863.1 XM_017004864.1 XM_017004865.1 XM_017004866.1

Validation Results:

19%(+/-0% mRNA remaining)(HeLa cell line)

Validation Procedure:

The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.