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SiRNA Validation Data

siRNA ID:

974

Gene ID:

22858

Transcript(s):

NM_014920.3 NM_016513.4 XM_011514419.1 XM_011514420.2 XM_011514421.1 XM_017010485.1 XM_017010486.1 XM_017010487.1 XM_017010488.1 XM_017010489.1 XM_017010490.1 XM_017010491.1 XM_017010492.1

Validation Results:

18%(+/-0% mRNA remaining)(HeLa cell line)

Validation Procedure:

The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.