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SiRNA Validation Data

siRNA ID:

985

Gene ID:

50488

Transcript(s):

NM_001024937.3 NM_001321236.1 NM_015716.4 NM_153827.4 NM_170663.4 XM_006721531.2 XM_006721532.2 XM_006721536.2 XM_011523906.1 XM_017024704.1 XM_017024705.1 XM_017024706.1 XM_017024707.1 XM_017024708.1

Validation Results:

21%(+/-0% mRNA remaining)(HeLa cell line)

Validation Procedure:

The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.