SiRNA Validation Data

siRNA ID:

s10740

Gene ID:

5395

Transcript(s):

NM_000535.6 NM_001322003.1 NM_001322004.1 NM_001322005.1 NM_001322006.1 NM_001322007.1 NM_001322008.1 NM_001322009.1 NM_001322010.1 NM_001322011.1 NM_001322012.1 NM_001322014.1 NM_001322015.1 NR_136154.1 XM_017012342.1

Validation Results:

17%(+/-4% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00241053_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.