SiRNA Validation Data

siRNA ID:

s10884

Gene ID:

5467

Transcript(s):

NM_001171818.1 NM_001171819.1 NM_001171820.1 NM_006238.4 NM_177435.2 XM_005249193.1 XM_006715120.1 XM_006715123.1 XM_011514707.1 XM_011514709.1 XM_011514710.1 XM_017010971.1 XM_017010972.1 XM_017010973.1 XM_017010974.1 XM_017010975.1

Validation Results:

15%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00606407_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.