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SiRNA Validation Data

siRNA ID:

s11175

Gene ID:

5606

Transcript(s):

NM_001316332.1 NM_002756.4 NM_145109.2 XM_005256723.2 XM_011523958.2 XM_011523959.1 XM_017024857.1 XM_017024858.1 XM_017024859.1

Validation Results:

13%(+/-4% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00177127_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.