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SiRNA Validation Data

siRNA ID:

s12281

Gene ID:

6197

Transcript(s):

NM_004586.2 XM_005274573.2 XM_005274577.2 XM_006724507.3 XM_011545555.1 XM_011545556.1 XM_011545557.2 XM_011545558.2 XM_011545559.1 XM_011545560.1 XM_011545561.1 XM_011545562.1 XM_011545563.2 XM_017029713.1 XM_017029714.1 XM_017029715.1 XM_017029716.1 XM_017029717.1 XM_017029718.1 XM_017029719.1

Validation Results:

6%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00177936_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer^® Select siRNA or 5 nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.