SiRNA Validation Data

siRNA ID:

s14972

Gene ID:

7534

Transcript(s):

NM_001135699.1 NM_001135700.1 NM_001135701.1 NM_001135702.1 NM_003406.3 NM_145690.2 XM_005251061.3 XM_005251063.3 XM_017013810.1 XM_017013811.1

Validation Results:

3%(+/-0% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00237047_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.