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SiRNA Validation Data

siRNA ID:

s21887

Gene ID:

11101

Transcript(s):

NM_001001976.2 NM_001288734.1 NM_001288735.1 NM_001288736.1 NM_007041.3 NR_110006.1 XM_005269458.2 XM_005269459.3 XM_017015597.1 XM_017015598.1 XM_017015599.1 XM_017015600.1 XM_017015602.1 XM_017015604.1

Validation Results:

12%(+/-5% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00379067_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.