SiRNA Validation Data

siRNA ID:

s26492

Gene ID:

29102

Transcript(s):

NM_001100412.1 NM_013235.4 XM_005248291.3 XM_005248292.3 XM_005248293.3 XM_005248294.3 XM_011514033.2 XM_017009399.1 XM_017009400.1 XM_017009401.1 XM_017009402.1

Validation Results:

14%(+/-4% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00294820_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.