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SiRNA Validation Data

siRNA ID:

s27006

Gene ID:

50488

Transcript(s):

NM_001024937.3 NM_001321236.1 NM_015716.4 NM_153827.4 NM_170663.4 XM_006721531.2 XM_006721532.2 XM_006721536.2 XM_011523906.1 XM_017024704.1 XM_017024705.1 XM_017024706.1 XM_017024707.1 XM_017024708.1

Validation Results:

20%(+/-6% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00179553_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer^® Select siRNA or 5 nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.