SiRNA Validation Data

siRNA ID:

s27221

Gene ID:

51006

Transcript(s):

NM_001281457.1 NM_001281458.1 NM_001281459.1 NM_001281460.1 NM_015945.11 NM_173073.3 NM_173179.3 XM_011528831.1 XM_011528832.1 XM_011528833.1 XM_011528834.1 XM_011528836.1 XM_011528837.1 XM_017027856.1 XM_017027857.1 XM_017027858.1 XM_017027859.1 XM_017027860.1 XM_017027861.1 XM_017027862.1

Validation Results:

5%(+/-0% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00210988_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.