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SiRNA Validation Data

siRNA ID:

s2759

Gene ID:

996

Transcript(s):

NM_001114091.2 NM_001256.4 NM_001293089.1 NM_001293091.1 XM_011525546.2 XM_011525547.2 XM_011525548.2 XM_011525549.2 XM_011525550.2 XM_017025481.1 XM_017025482.1 XM_017025483.1 XM_017025484.1 XM_017025485.1 XM_017025486.1 XM_017025487.1 XM_017025488.1

Validation Results:

10%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00265810_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer^® Select siRNA or 5 nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.