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SiRNA Validation Data

siRNA ID:

s28813

Gene ID:

53938

Transcript(s):

NM_032472.3 NM_130906.2 XM_005246651.3 XM_005246652.4 XM_011511358.2 XM_017004352.1 XM_017004353.1 XM_017004354.1 XM_017004355.1 XM_017004356.1 XM_017004357.1 XM_017004358.1 XM_017004359.1 XM_017004360.1

Validation Results:

6%(+/-0% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00368985_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.