SiRNA Validation Data

siRNA ID:

s32717

Gene ID:

57118

Transcript(s):

NM_020397.3 NM_153498.3 XM_006717481.3 XM_006717482.3 XM_006717483.3 XM_011519591.2 XM_011519592.2 XM_011519593.2 XM_011519594.2 XM_011519595.2 XM_017016438.1 XM_017016439.1

Validation Results:

18%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00220668_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.