SiRNA Validation Data

siRNA ID:

s3698

Gene ID:

1487

Transcript(s):

NM_001012614.1 NM_001328.2 XM_005272261.3 XM_005272263.4 XM_011513395.2 XM_011513396.2 XM_011513397.2 XM_017007762.1 XM_017007763.1 XM_017007764.1 XM_017007765.1 XM_017007766.1 XM_017007767.1

Validation Results:

8%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00179922_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.