SiRNA Validation Data

siRNA ID:

s37704

Gene ID:

81614

Transcript(s):

NM_001008860.2 NM_001008892.2 NM_001008894.2 NM_001184888.1 NM_001184889.1 NM_030922.6 XM_005272546.3 XM_005272547.4 XM_005272548.3 XM_005272550.3 XM_005272552.3 XM_005272553.4 XM_006720364.2 XM_006720365.2 XM_006720366.3 XM_006720367.1 XM_011543877.2 XM_011543878.2 XM_011543879.2 XM_011543880.2 XM_017022645.1 XM_017022646.1 XM_017022647.1 XM_017022648.1 XM_017022649.1 XM_017022650.1 XM_017022651.1 XM_017022652.1 XM_017022653.1 XM_017022654.1 XM_017022655.1 XM_017022656.1 XM_017022657.1 XM_017022658.1 XM_017022659.1 XM_017022660.1 XM_017022661.1 XM_017022662.1 XM_017022663.1

Validation Results:

3%(+/-0% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00384478_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.