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SiRNA Validation Data

siRNA ID:

s4400

Gene ID:

1859

Transcript(s):

NM_001396.3 NM_101395.2 NM_130436.2 NM_130438.2 XM_005260933.4 XM_006723976.3 XM_006723977.3 XM_006723978.3 XM_006723979.3 XM_011529482.2 XM_011529483.2 XM_011529484.2 XM_011529485.2 XM_017028284.1 XM_017028285.1 XM_017028286.1

Validation Results:

17%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00176369_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.