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SiRNA Validation Data

siRNA ID:

s7068

Gene ID:

3371

Transcript(s):

NM_002160.3 XM_005251972.3 XM_005251973.3 XM_005251974.3 XM_005251975.3 XM_006717096.3 XM_006717097.3 XM_006717098.3 XM_006717101.3 XM_011518625.2 XM_011518626.2 XM_011518628.2 XM_011518629.2 XM_017014678.1 XM_017014679.1 XM_017014680.1 XM_017014681.1

Validation Results:

4%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00233648_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.