SiRNA Validation Data

siRNA ID:

s8792

Gene ID:

4286

Transcript(s):

NM_000248.3 NM_001184967.1 NM_001184968.1 NM_006722.2 NM_198158.2 NM_198159.2 NM_198177.2 XM_005264754.1 XM_005264755.3 XM_006713164.2 XM_011533722.2 XM_011533723.2 XM_017006444.1 XM_017006445.1 XM_017006446.1 XM_017006447.1 XM_017006448.1

Validation Results:

13%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs01117294_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.