SiRNA Validation Data

siRNA ID:

105832

Gene ID:

Transcript(s):

NM_001161725.1 NM_001161726.1 NM_002719.3 NM_178586.2 NM_178587.2 XM_005267819.1 XM_005267820.1 XM_005267822.3 XM_005267823.1 XM_005267824.1 XM_005267826.1 XM_005267827.1 XM_011536914.2 XM_011536915.2 XM_011536916.2 XM_011536918.1 XM_011536919.2 XM_011536920.1 XM_011536921.2 XM_017021422.1 XM_017021423.1 XM_017021424.1

Validation Results:

6%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00604902_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.