SiRNA Validation Data

siRNA ID:

1430

Gene ID:

9448

Transcript(s):

NM_001242559.1 NM_001242560.1 NM_004834.4 NM_145686.3 NM_145687.3 XM_005264048.2 XM_005264050.3 XM_005264052.3 XM_005264057.3 XM_005264058.3 XM_005264059.3 XM_005264060.3 XM_005264062.3 XM_005264063.3 XM_005264064.3 XM_005264065.3 XM_005264066.3 XM_005264068.3 XM_005264069.2 XM_005264071.2 XM_006712865.2 XM_006712868.2 XM_006712869.2 XM_017005349.1 XM_017005350.1 XM_017005351.1 XM_017005352.1 XM_017005353.1 XM_017005354.1 XM_017005355.1 XM_017005356.1 XM_017005357.1 XM_017005358.1 XM_017005359.1 XM_017005360.1 XM_017005361.1 XM_017005362.1 XM_017005363.1 XM_017005364.1 XM_017005365.1 XM_017005366.1 XM_017005367.1 XM_017005368.1 XM_017005369.1 XM_017005370.1 XM_017005371.1 XM_017005372.1 XM_017005373.1 XM_017005374.1 XM_017005375.1

Validation Results:

23%(+/-11% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00377410_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.