SiRNA Validation Data

siRNA ID:

1442

Gene ID:

Transcript(s):

NM_002037.5 NM_153047.3 NM_153048.3 XM_005266890.3 XM_005266892.3 XM_017010650.1 XM_017010651.1 XM_017010652.1 XM_017010653.1 XM_017010654.1 XM_017010655.1

Validation Results:

16%(+/-5% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00176628_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.