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SiRNA Validation Data

siRNA ID:

1497

Gene ID:

79705

Transcript(s):

NM_024652.4 XM_005254979.3 XM_011522012.2 XM_011522013.2 XM_011522014.2 XM_011522015.2 XM_011522016.2 XM_011522017.2 XM_011522018.2 XM_017022570.1

Validation Results:

13%(+/-7% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00226465_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.