SiRNA Validation Data

siRNA ID:

s20925

Gene ID:

10645

Transcript(s):

NM_001270485.1 NM_001270486.1 NM_006549.3 NM_153499.2 NM_153500.1 NM_172214.2 NM_172215.2 NM_172216.1 NM_172226.2 XM_005253822.3 XM_005253823.1 XM_005253824.3 XM_011537763.1 XM_011537764.2 XM_017018694.1 XM_017018695.1 XM_017018696.1 XM_017018697.1 XM_017018698.1 XM_017018699.1 XM_017018700.1 XM_017018701.1 XM_017018702.1 XM_017018703.1 XM_017018704.1 XM_017018705.1 XM_017018706.1 XM_017018707.1 XM_017018708.1 XM_017018709.1

Validation Results:

12%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00198032_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.