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SiRNA Validation Data

siRNA ID:

s23755

Gene ID:

23405

Transcript(s):

NM_001195573.1 NM_001271282.2 NM_001291628.1 NM_030621.4 NM_177438.2 XM_011536599.1 XM_011536600.2 XM_011536601.2 XM_011536602.2 XM_011536604.2 XM_011536605.2 XM_017021120.1 XM_017021121.1 XM_017021122.1 XM_017021123.1

Validation Results:

20%(+/-0% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00229023_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.