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SiRNA Validation Data

siRNA ID:

s5720

Gene ID:

2677

Transcript(s):

NM_000821.6 NM_001142269.3 XM_005264259.4 XM_011532764.2 XM_011532765.2 XM_017003803.1

Validation Results:

7%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00168139_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.