SiRNA Validation Data

siRNA ID:

105113

Gene ID:

Transcript(s):

NM_001272075.1 NM_005153.2 NR_073578.1 XM_006721332.1 XM_011523440.1 XM_011523441.1 XM_011523443.1 XM_017023863.1 XM_017023864.1 XM_017023865.1 XM_017023866.1 XM_017023867.1 XM_017023868.1 XM_017023869.1

Validation Results:

14%(+/-6% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00382487_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.