SiRNA Validation Data

siRNA ID:

1109

Gene ID:

Transcript(s):

NM_001172303.2 NM_001172304.2 NM_001320756.1 NM_001320757.1 NM_032844.4 NR_135469.1 XM_005252631.3 XM_005252632.3 XM_006717519.3 XM_006717520.3 XM_017016852.1 XM_017016853.1 XM_017016854.1 XM_017016855.1 XM_017016856.1

Validation Results:

11%(+/-4% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00262532_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.