SiRNA Validation Data

siRNA ID:

1174

Gene ID:

65125

Transcript(s):

NM_001184985.1 NM_014823.2 NM_018979.3 NM_213655.4 XM_006719003.2 XM_011520997.2 XM_011520998.2 XM_011520999.2 XM_011521000.2 XM_011521001.2 XM_011521002.2 XM_011521003.2 XM_011521004.2 XM_011521006.2 XM_011521007.2 XM_011521008.2 XM_011521009.2 XM_017019834.1 XM_017019835.1 XM_017019836.1 XM_017019837.1 XM_017019838.1 XM_017019839.1

Validation Results:

8%(+/-0% mRNA remaining)(HeLa cell line)

Validation Procedure:

The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.