SiRNA Validation Data

siRNA ID:

119070

Gene ID:

410

Transcript(s):

NM_000487.5 NM_001085425.2 NM_001085426.2 NM_001085427.2 NM_001085428.2 XM_011530690.2 XM_011530691.2 XM_017028800.1

Validation Results:

18%(+/-5% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00166646_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.