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SiRNA Validation Data

siRNA ID:

121124

Gene ID:

118424

Transcript(s):

NM_058167.2 NM_194315.1 NM_194457.1 NM_194458.1 XM_005244718.3 XM_005244719.4 XM_006710333.3 XM_011540613.2 XM_011540614.2 XM_011540616.2 XM_011540617.2 XM_017000238.1 XM_017000239.1

Validation Results:

8%(+/-5% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00377929_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.